NT5E polyclonal antibody
产品名称： NT5E polyclonal antibody
英文名称： NT5E polyclonal antibody
产品编号： PAB3845
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of NT5E.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NT5E.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts.
Hashikawa T, Takedachi M, Terakura M, Saho T, Yamada S, Thompson LF, Shimabukuro Y, Murakami S.J Dent Res. 2003 Nov;82(11):888-92.
- 2.
- Ecto-5'-nucleotidase in B-cell chronic lymphocytic leukemia.
Rosi F, Carlucci F, Marinello E, Tabucchi A.Biomed Pharmacother. 2002 Mar;56(2):100-4.
- 3.
- Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.
Misumi Y, Ogata S, Ohkubo K, Hirose S, Ikehara Y.Eur J Biochem. 1990 Aug 17;191(3):563-9.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of NT5E polyclonal antibody (Cat # PAB3845) in Y-79 cell line lysate (35 ug/lane). NT5E (arrow) was detected using the purified polyclonal antibody.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with NT5E polyclonal antibody (Cat # PAB3845) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
- Entrez GeneID:
- 4907
- Protein Accession#:
- NP_002517;P21589
- Gene Name:
- NT5E
- Gene Alias:
- CD73,E5NT,NT,NT5,NTE,eN,eNT
- Gene Description:
- 5'-nucleotidase, ecto (CD73)
- Omim ID:
- 129190
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
- Other Designations:
- 5' nucleotidase (CD73),5' nucleotidase, ecto,OTTHUMP00000016808,OTTHUMP00000040565,Purine 5-Prime-Nucleotidase,ecto-5'-nucleotidase